Twenty-five years of quantitative PCR for gene expression analysis.

short Communication: Optimization of semi-quantitative RT PCR analysis for CPT I gene expression in Rainbow trout (Oncorhynchus mykiss)

Quantitative expression analysis of candidate genes for Septoria tritici blotch resistance in wheat (Triticum aestivum L.)

Septoria tritici blotch (STB), caused by the ascomycete fungus Mycosphaerella graminicola (asexual stage: Septoria tritici), is one of the most important foliar diseases of wheat. In this research, quantitative expression analysis of five candidate genes for the induction of resistance to STB (PR-1, Bsi, Msr, Per, and Ppi) was conducted in the wheat cultivars ‘Seri 82’ (susceptible) and ‘Fronta...

short Communication: Optimization of semi-quantitative RT PCR analysis for CPT I gene expression in Rainbow trout (Oncorhynchus mykiss)

Semiquantitative RT-PCR analysis to assess the expression levels of iNOS in chicken macrophages

Abstract: iNOS is inducible by a variety of factors related to inflammation and referred to as inducible NOS(iNOS). It is regulated at the level of gene expression; once expressed, it produces NO at a high rate. iNOS gene-expression profiling is an important tool in understanding molecular markers of the responses of cells and tissues to external factors. In this article a semiquantitative reve...

Validation of a genus-specific gene; TPS, used as internal control in quantitative Real Time PCR of transgenic cotton

Identification of genes with invariant levels of gene expression is a prerequisite for validating transcriptomic changes accompanying development. Ideally expression of these genes should be independent of the morphogenetic process or environmental condition.We report here the validation of internal control gene i.e.TPS (trehalose 6-phosphate-synthase) in cotton (Gossypium spp), using TaqMan sy...

Expression pattern analysis of TomPRO2 and LaPA1 genes in tomato under in vitro salt stress by Semi-quantitative RT-PCR

The expression pattern of TomPRO2 and LaPA1 genes in two tomato (Lycopersicon esculentum) cultivars named as Isfahani and Shirazi under in vitro salt stress were investigated. Four to six weeks old in vitro grown seedlings were transferred on MS medium containing 0, 80 and 160 mM NaCl and untreated plants were used as control. RNA was extracted from root and leaf and then cDNA was synthesized. ...